Rapid Synthesis of Nucleoside Triphosphates and Analogues

Enzymatic synthesis of nucleoside diphosphates and triphosphates.

It has been recognized from recent studies that nucleoside triphosphates, other than ATP,’ function in biologic systems as phosphorylating agents (1) and in coenzyme synthesis (2) in a manner analogous to that of ATP. The discovery of the natural occurrence of a variety of purine and pyrimidine nucleoside diand triphosphates (3-5) and the likelihood that new biologic functions for these nucleot...

Chemical Synthesis of Nucleoside Analogues Chemical Synthesis of Nucleoside Analogues

Nucleoside triphosphates--from synthesis to biochemical characterization.

The traditional strategy for the introduction of chemical functionalities is the use of solid-phase synthesis by appending suitably modified phosphoramidite precursors to the nascent chain. However, the conditions used during the synthesis and the restriction to rather short sequences hamper the applicability of this methodology. On the other hand, modified nucleoside triphosphates are activate...

Enzymatic synthesis of nucleoside S-mono and -triphosphates

Recently we have reported on the chemical synthesis of dNTP(3’F) and NTP(3’F) and their use as terminators of the biosynthesis of DNA and RNA, respectively [l-4]. During the course of the chemical synthesis of fluorodeoxyriboand 2 ’ -deoxyribo-NMP and NTP, we have pointed out that the isolated yields of pure compounds are varied within the broad limits depending on the structure of a heterocycl...

Glycerol nucleoside triphosphates: synthesis and polymerase substrate activities.

[Structure: see text] The synthesis of (S)-glycerol nucleoside triphosphates (gNTPs) and the analysis of their substrate activities for enzymatic polymerization is described. NTPs with simplified carbohydrate backbones such as the tNTPs (alpha-L-threose-NTPs) are polymerase substrates and offer the potential to create non-natural aptamer sequences with simplified backbones through enzymatic mea...